Grimsby Town fell to back-to-back league defeats as Carlisle United took all three points away from Blundell Park.
In an open first half, both sides created chances and visiting keeper Jack Bonham produced a string of excellent saves to keep the Mariners out.
And the hosts were punished for missing their opportunities when veteran defender Clint Hill grabbed what turned out to be the only goal of the game 20 minutes from time.
Four changes were made to the Town starting XI after the defeat at Exeter City last time out, with Zak Mills, Mitch Rose, Diallang Jaiyesimi and JJ Hooper coming in for Paul Dixon, James Berrett, Martyn Woolford and Sam Jones.
James McKeown started in goal, with Mills coming in at right-back as Ben Davies moved across to the left, with Nathan Clarke and Danny Collins completing the defence.
Siriki Dembele, Rose, Luke Summerfield and Jaiyesimi were the midfielders, with Hooper joining Scott Vernon in attack.
Jones was named among the substitutes, along with Ben Killip, Sam Kelly, Jamey Ossborne, Karleigh Osborne, Harry Cardwell and Jamille Matt.
Carlisle made the long trip from the north-west level on points with the Mariners in the League Two table, wih the Cumbrians' better goal difference seeing them one place higher in the standings.
Manager Keith Curle would look to the likes of former England youth international Hallam Hope to cause problems for the home defence, while the experienced Hill was among those aiming to limit chances at the other end.
Summerfield gave away a corner on three minutes which provided the first attacking opportunity of the game but when the ball was played into the penalty area from it, the midfielder was there to clear.
A free kick soon afterwards for a foul on Reggie Lambe was then driven towards goal by Carlisle captain Danny Grainger but McKeown got down to push the ball away from danger.
At the other end, a low, left-wing cross from Davies was caught by Bonham, before striker Richie Bennett narrowly failed to connect with a cross after a good attacking move from the away side.
Town twice went close to taking the lead on nine minutes, when Vernon was denied by a good save from Bonham and then Jaiyesimi hit the bar with a shot from close range.
Dembele was the next to have a chance as the Mariners continued to attack, with keeper Bonham again coming off his line to block the winger's shot.
Lambe won a corner on the Carlisle left on 18 minutes and after the ball from the flag was cleared, midfielder Luke Joyce hit a long-range effort that was deflected out for another corner. That turned into a third in quick succession and after the ball was punched clear by McKeown, it was worked back for Bennett to head wide.
A run from Jaiyesimi looked to have created an opportunity for Town two minutes later but after the ball was played to Vernon, he could only send a weak effort through to Bonham.
And another chance came when a Caarlisle shot was charged down and the ball was picked up by Hooper, who made progress down the left before cutting in and curling a low shot wide of the far post.
Dembele let fly from 30 yards on 24 minutes but his shot was straight at the keeper, with Carlisle next to threaten just after the half-hour, although nobody could get on the end of Hope's low cross.
Summerfield sent a shot over the bar from 25 yards on 35 minutes, then a cross frrom Davies on the left looked like it might be heading towards goal but Bonham was able to watch it go over the bar.
A free kick in a central position and midway inside the Carlisle half gave Summerfield another opportunity to shoot but his powerful effort was straight at Bonham, who gathered the ball at the second attempt.
A push on Davies by Kelvin Etuhu brought about another free kick, this time on the left. Summerfield's ball from it was pushed away for a corner by Bohnam, who then made a superb save from a Collins header which looked destined for the net after the defender had met the flag-kick.
McKeown was called into action at the other end on 43 minutes, with the keeper making a good block after Bennett had found himself with a chance at the far post.
One additional minute was signalled as Lambe went through on goal for Carlisle but could only slide the ball wide of the near post.
And that proved to be the final opportunity for either side to break the deadlock before the interval as the first half ended goalless.
No changes were made before the restart and an early free kick frrom Summerfield on the left caused no problems as it beat everybody and went out for a goal kick.
A good attacking move from the hosts led to the ball being played to Mills out on the right but after he crossed, Hooper could only divert the ball wide of the near post.
Good persistence from Jaiyesimi on the left eventually led to him getting a chance with a volley frrom inside the box but it did not trouble Bonham as the ball flew over the bar.
Another long-range shot from Summerfield was just too high, before former Town loanee Jamie Devitt eventually replaced Bennett after the Carlisle manageement team had initially decided against making the change.
McKeown had to tip the ball over his bar fom an Etuhu header on 58 minutes but the resulting corner came to nothing.
Devitt hit the roof of the Osmond Stand with a shot from 25 yards, before Jones replaced Hooper in Grimsby's first substitution of the afternoon.
A free kick from the Town left was dealt with after Summerfield's delivery into the box, before Clarke had to head the ball out for a corner at the other end to prevent Hope from connecting with a crross.
That defensive header kept the score goalless but it was not to stay that way for long, as the visitors capitalised on space on the right and worked the ball through for HILL to head into the net at the near post and open the scoring.
Matt was sent on in place of Vernon with the Mariners now chasing the game and less than 20 minutes left to play.
But it was Carlisle who were doing most of the attacking and they had another corner on 77 minutes, which was headed clear by Clarke.
McKeown saved with his feet after a free kick was driven in from the visitors' right, before Jamey Osborne was sent on for the last 11 minutes of the game in place of Jaiyesimi.
Mills coul only find keeper Bonham with a low shot from 25 yards as the Mariners looked for a way back into a contest which seemed to be slipping away from them.
Four added minutes were signalled as Town pushed for a late equaliser but it was not to come and instead, they were left to reflect on a first home defeat in the league since Wycombe Wanderers took all three points in August.
It was also a fourth consecutive home game without a goal for the Mariners, who will look to put that statistic right when Swindon Town visit Blundell Park on Tuesday.
1 James McKeown
2 Ben Davies
12 Zak Mills
6 Danny Collins
5 Nathan Clarke (C)
19 Luke Summerfield
27 Siriki Dembele
8 Mitch Rose
20 Diallang Jaiyesimi 80' <
9 JJ Hooper 65' <
21 Scott Vernon 74' <
30 Ben Killip
14 Karleigh Osborne
10 Sam Jones 65' >
11 Sam Kelly
16 Jamey Osborne 80' >
17 Harry Cardwell
29 Jamille Matt 74' >
1 Jack Bonham
3 Danny Grainger (C)
17 James Brown
29 Clint Hill
5 Gary Liddle
4 Luke Joyce
21 Kelvin Etuhu
8 Michael Jones
14 Richard Bennett
19 Reggie Lambe
9 Hallam Hope
23 Shamal George
2 Tom Miller
6 Tom Parkes
11 Jamie Devitt
18 John O'Sullivan
15 Sam Cosgrove
20 Shaun Miller
Referee: Robert Lewis
Attendance: 3753 (218)