Town face their second Lincolnshire derby in the space of four days when they head to Glanford Park to take on Scunthorpe United in the Checkatrade Trophy on Tuesday night (7:45pm).
After Saturday's goalless draw at home to Lincoln City, Russell Slade's attentions will turn to the Checkatrade Trophy, where Town will hoping for a victory after losing on penalties to Doncaster
Although changes are likely to be made by both sides, it is always a tense occasion when the Mariners and the Iron meet each other and that will be no different this time around.
Mills set to return
Zak Mills could return from injury to take his place in the starting line-up with Russell Slade admitting his pre-match press conference that he hopes to give the defender some minutes.
Karleigh Osborne and Harry Clifton could also come into contention for some playing time, whilst Luke Summerfield and Ben Davies will miss out after picking up knocks at the weekend.
Scunthorpe boss Graham Alexander also admitted that he would like to give some players game time, but maintained that his players will be motivated to take advantage of their chance in the side.
A good start for Scunthorpe
Scunthorpe United have been in the upper echeleons of League One ever since Graham Alexander took over, and they are just outside the play-offs in 7th place going into the second round of Checkatrade Trophy games.
The Iron beat Sunderland U21 in the first round of fixtures, wih goals from Duana Holmes, Kevin Van Veen and Josh Morris, so they will be looking to seal their place in the next round here with another three points.
Manager Alexander took over in March 2016 and has put together a good squad, which means the Mariners could come up against the likes of exciting winger Hakeeb Adelakun, Dutch striker Van Veen and former Republic of Ireland international Paddy Madden on Tuesday night.
A memorable cup tie last time
The last competitive meeting between the two sides came in the FA Cup first round back in November 2013.
A goalless draw in the first fixture at Blundell Park meant a replay at Glanford Park, and it was there that goals from Lenell John-Lewis and Clayton McDonald saw the Mariners to a memorable victory.
The Mariners have met the Iron on 56 occasions over the years, with Town winning 25 and Scunthorpe winning 14 along with 17 draws.
Managers in summary
Russell Slade: ''I think obviously Scunthorpe a bit more established in League One at this moemnt in time and will probably be looking to get in that top seven so it will be a very tough game, they've got some very good players and established League One players so we expect whatever team he puts out, and he has got a strong squad at his disposal, for it to be a tough game. It's a really good opportunity for players that haven't been playing to come in and they're all valued players, so I'm hoping they can give me a headache really before Saturday.''
Graham Alexander: ''We played against Notts County, who are flying high in League Two in the Carabao Cup and saw what a tough game that was. We never underestimate any challenge we’ve got - we didn’t against Sunderland Under-21s and I thought that was a competitive game as well. We want to be on the front foot and want to feel good about ourselves when we come off the pitch. The only way to do that is by playing well and winning. We want to do well in this competition.''